Hemoglobin function in stored blood

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Hemoglobin Function in Stored Blood

INTRODUCTION The ultimate aim in transfusing blood is the maintenance or restoration of a medium for the transport of oxygen to body tissues. Most often, the efficacy of transfusion therapy is evaluated by survival of the donor erythrocytes in the recipient. However, red cells may have a normal in vivo survival yet be unable to bind and unload oxygen physiologically. In 1956, Valtis and Kennedy...

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Iron accelerates hemoglobin oxidation increasing mortality in vascular diseased guinea pigs following transfusion of stored blood.

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Multiphoton excited hemoglobin fluorescence and third harmonic generation for non-invasive microscopy of stored blood.

Red blood cells (RBC) in two-photon excited fluorescence (TPEF) microscopy usually appear as dark disks because of their low fluorescent signal. Here we use 15fs 800nm pulses for TPEF, 45fs 1060nm pulses for three-photon excited fluorescence, and third harmonic generation (THG) imaging. We find sufficient fluorescent signal that we attribute to hemoglobin fluorescence after comparing time and w...

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Posttransfusional recovery of defective respiratory function of stored blood in dogs.

Changes in the oxygenation properties and the relevant biochemical parameters of canine blood were followed during its storage in acid-citrate-dextrose (ACD) medium (4 degrees C) and after transfusion of the stored blood. Reductions of the blood P50 (pH 7.40, PCO2 40 Torr, 37 degrees C) and 2,3-diphosphoglycerate (DPG) during storage was much slower than in human blood. Half-decay time for DPG ...

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ژورنال

عنوان ژورنال: Journal of Clinical Investigation

سال: 1969

ISSN: 0021-9738

DOI: 10.1172/jci105987